為什么使用單板培養(yǎng)箱?  

細(xì)胞生長需要穩(wěn)定和無菌的環(huán)境條件。 培養(yǎng)箱允許細(xì)胞在可控和受保護(hù)的環(huán)境中發(fā)育。 由于設(shè)計(jì)緊湊和培養(yǎng)箱的最小尺寸，單板培養(yǎng)箱可以非常迅速地補(bǔ)償環(huán)境條件中與工藝相關(guān)的波動(例如，當(dāng)門打開或關(guān)閉時(shí))。 這樣，細(xì)胞受到的壓力就可以降到最低。 

單盤培養(yǎng)箱有什么好找的  

孵化器可在許多不同的配置，但溫度斜坡速率和準(zhǔn)確性加上保護(hù)從光和污染是必不可少的。 以下是一些需要考慮的主要因素。  

尺寸和容量-與處理樣品在不同的細(xì)胞載體的能力，而適合您的工作站舒適。 對所有實(shí)驗(yàn)室來說，規(guī)模越大未必就越好。  

基于地板或工作臺頂部-基于地板往往有更大的容量，工作臺頂部可能更容易集成到您的工作流程。  

可編程性/自動化-你的孵化器如何與你現(xiàn)有的設(shè)置集成?  

溫度范圍-您的化驗(yàn)可能需要的最高溫度是多少?  

溫度精度和均勻性-對所有檢測的精確處理至關(guān)重要。  

溫度漸變率-你的細(xì)胞獲得溫度的速度越快，你的吞吐量可以更高。  

清潔和消毒——為了保持樣品不被污染，培養(yǎng)箱應(yīng)該容易保持清潔。  

使用INHECO單板培養(yǎng)箱  

INHECO單平板培養(yǎng)箱在世界各地的實(shí)驗(yàn)室中用于ngs文庫制備、PCR、孵育和連續(xù)酶解。 

它通常與液體處理工作站、閱讀器和機(jī)械臂相結(jié)合。 

實(shí)驗(yàn)室還使用INHECO單板培養(yǎng)箱進(jìn)行ELISA、生物測定、NA雜交和暗培養(yǎng)，以保護(hù)敏感(熒光)染料不被降解。

Why use a single plate incubator?

Cells need stable and sterile environmental conditions for optimal cell growth. An incubator allows cells to develop in a controlled and protected environment. Due to the compact design and the minimal size of the incubation chamber, single-plate incubators can very quickly compensate for process-related fluctuations in ambient conditions (e.g. when the door is opened or closed). The stress on the cells can thus be minimized.

What to look for in a single plate incubator

Incubators are available in many different configurations, but temperature ramp rate and accuracy plus protection from light and contamination are essential to all. Here are some of the main factors to consider.

Size and capacity – with the ability to handle samples in varying cell carriers while fitting comfortably into your workstation. Bigger is not necessarily better for all labs.

Floor-based or bench top – floor-based tend to have larger capacity, bench top may be easier to integrate into your workflow.

Programmability/automation – how will your incubator integrate with your existing set up?

Temperature range – what is the hottest your assays are likely to require?

Temperature accuracy and uniformity – essential for precise processing of all your assays.

Temperature ramp rate – the faster your cells get to temperature, the higher your throughput can be.

Cleaning and sterilizing – to keep your samples uncontaminated, your incubator should be easy to keep clean.

Use of the INHECO Single Plate Incubator

The INHECO Single Plate Incubator is used in NGS-library preparation, PCR, incubation and sequential enzymatic digestions in labs around the world.

It’s often integrated with liquid handling workstations, in combination with a reader and robotic arm.

Labs also use the INHECO Single Plate Incubator for ELISA, bioassays, NA Hybridization and dark incubation to protect sensitive (fluorescent) dyes from degradation.