LE Magnet Plate

Low Elution Magnet Plate低洗脫磁力架 SKU: A000350

LE Magnet Plate quantity 

Used with 96-well PCR plates when low elution volumes are needed to ensure high product concentration. This plate allows you to elute in as little as 8-10 μl.

LE磁力架數(shù)量

用于96孔PCR板時(shí)，需要低洗脫量，以確保高的產(chǎn)物濃度。該板允許您在少至8-10 μ l洗脫。

When concentrated nucleic acids are required in downstream applications

Minimize reaction volume, maximize product concentration.

Elute in as little as 8 μL

下游應(yīng)用中需要濃縮核酸時(shí)

最小化反應(yīng)量，最大化產(chǎn)品濃度。

洗脫僅需8 μL

We are using this plate on our liquid handler for NGS purposes and works very well in our hands. We were able to lower volumes and thus reduce costs without loss of quality and repetitiveness.

So an absolute recommendation.

– Ewart de Bruijn

我們正在使用這個(gè)板在我們的液體處理程序?yàn)镹GS的目的，在我們的手中工作得很好。我們能夠降低產(chǎn)量，從而在不降低質(zhì)量和重復(fù)性的情況下降低成本。

所以絕對(duì)推薦。

- Ewart de Bruijn

Maximize product concentration.

Unique magnet architecture for very low volume sample elution.

The LE magnet is on its way to being my favorite of the four different types I have to choose from in my lab. I have had no issues using it with single and multichannel pipettes down to as little as 7.5uL elution volumes. Separation times are super fast with low volumes (<20s, generally), and I don’t see any carry over.

Highly recommended.

– Peter

最大化產(chǎn)品濃度。

獨(dú)特的磁鐵結(jié)構(gòu)，非常低體積的樣品洗脫。

在我的實(shí)驗(yàn)室里，有四種不同類型的磁鐵可供我選擇，而LE磁鐵正逐漸成為我最喜歡的一種。我在單通道和多通道移液器中使用它沒(méi)有任何問(wèn)題，低至7.5uL的洗脫量。分離時(shí)間非?？?，體積很小(一般小于20秒)，而且我沒(méi)有看到任何結(jié)轉(zhuǎn)。

強(qiáng)烈推薦。

——彼得

How does the LE Magnet Plate work?

The magnets used in the LE Magnet Plate have a smaller hole diameter than the 96R and 96S magnet plates. PCR wells don’t sit as deeply into these magnets, causing the ring of beads to form lower in the well. This means less elution volume is required (approximately 8-10 μl) to fully cover the beads and elute the product.

LE磁力架是如何工作的?

LE磁力架中使用的磁鐵具有比96R和96S磁鐵板更小的孔直徑。PCR孔并沒(méi)有深入到這些磁鐵中，導(dǎo)致珠環(huán)在孔的下部形成。這意味著需要更少的洗脫體積(約8-10 μ l)來(lái)完全覆蓋珠子和洗脫產(chǎn)品。

Full Working Volume

Full Working Volume, Fast Separation Times, Low Elution Volume

全工作容積

充分的工作體積，快速分離時(shí)間，低洗脫體積

Tests with a working solution of Ampure? XP (1 part water + 1.8 parts Ampure) have shown the following settle times:

Alpaqua LE Bead Separation Times

Reaction Volume Separation Time

100 μL 4 minutes

200 μL 6-7 minutes

300 μL < 10 minutes

Ampure?XP(1份水+ 1.8份Ampure)工作溶液的測(cè)試表明:

Alpaqua LE珠子分離時(shí)間

反應(yīng)體積分離時(shí)間

100 μ l 4分鐘

200 μ l 6-7分鐘

300 μ l < 10分鐘

Important Consideration for Optimum Results

Built For Precision Automated Liquid Handling: The LE Magnet Plate is intended for use with automated liquid handling equipment. Use with manual pipettors is not recommended.  It performs best in conjunction with an advanced liquid handling instrument equipped with a multi-channel dispense head, such as the Beckman Biomek? liquid handlers or the Eppendorf epMotion? platforms.  As a general rule, if your robot can safely work with 384 well PCR plates, the LE Magnet Plate should work well.

Only Compatible With Flat PCR Plates: It is very important to only use flat PCR plates with the LE Magnet Plate. All wells must touch the LE magnets so the ring of magnetic beads will form at the correct position in the wells. Do not use warped PCR plates because if the wells do not touch the LE magnets, the magnetic beads will cluster at the bottom and cause bead carry-over in those wells.  The LE is not for use with single PCR strips.

對(duì)最佳結(jié)果的重要考慮

為精密自動(dòng)化液體處理而建:LE磁鐵板用于自動(dòng)化液體處理設(shè)備。不建議與手動(dòng)移液器一起使用。它與配備多通道分配頭的先進(jìn)液體處理儀器配合使用，如Beckman Biomek?液體處理器或Eppendorf epMotion?平臺(tái)。一般來(lái)說(shuō)，如果你的機(jī)器人可以安全地使用384孔PCR板，那么LE磁鐵板應(yīng)該工作得很好。

只兼容平板PCR板:只使用平板PCR板和LE磁板是非常重要的。所有的井都必須接觸到LE磁鐵，這樣磁珠環(huán)就會(huì)在井中正確的位置形成。不要使用彎曲的PCR板，因?yàn)槿绻撞唤佑|LE磁鐵，磁珠會(huì)聚集在底部，導(dǎo)致珠在這些孔中攜帶。LE不用于單個(gè)PCR條帶。

Recommended PCR Microplates

The LE Magnet Plate works with most 96-well PCR plates – full-, semi-, or non-skirted.  The LE is not for use with single PCR strips.  Below are some common plates that we have confirmed reliably work.   Many others will work.  Please Contact Alpaqua if you would like to inquire about the compatibility of your specific PCR plate.

Eppendorf twin.tec 96 (full, semi, and non-skirted versions)

Abgene SuperPlate

Abgene AB-0800,  AB-1000 & Axygen PCR-96-FS-C

BioRad Hard-Shell Plate

推薦PCR微孔板

LE磁力架適用于大多數(shù)96孔PCR板-全，半，或非裙邊。LE不用于單個(gè)PCR條帶。下面是一些常見的板，我們已經(jīng)確認(rèn)可靠工作。其他許多方法也會(huì)奏效。如果您想查詢您的特定PCR板的兼容性，請(qǐng)聯(lián)系我們。

Eppendorf twin.tec 96(完整版、半版和無(wú)裙邊版)

Abgene SuperPlate

Abgene AB-0800, AB-1000 & Axygen PCR-96-FS-C

BioRad硬殼板 

Not Recommended: ABI MicroAmp? Fast 96-Well Reaction Plate, 0.1 mL

不推薦:ABI MicroAmp?快速96孔反應(yīng)板，0.1 mL