UNCOMPROMISING PURITY USING A MODULAR DESIGN AND INTUITIVE SOFTWARE WORKFLOW

不妥協(xié)的純度使用模塊化設(shè)計和直觀的軟件工作流程

Pre-programmed standard methods – use as is, or as a template for customized methods

Import sequences – easily create syntheses from focused libraries

Multi-task – plan one synthesis while another is running

Auto-calculations – reagent preparation and usage calculators built into the software Configurable on all vessel pairs – rapid, independent induction heating and real-time UV monitoring Upgradeable throughput – from 2 to 4 to 6 reaction vessels

預(yù)先編程的標準方法——按原樣使用，或者作為定制方法的模板  

導入序列-很容易從集中的庫創(chuàng)建合成  

多任務(wù)-計劃一個合成，當另一個合成正在運行  

自動計算-試劑制備和使用計算器內(nèi)置到軟件中可配置在所有容器對-快速，獨立的感應(yīng)加熱和實時紫外線監(jiān)測可升級的吞吐量-從2到4到6反應(yīng)容器

MULTI-SCALE   PEPTIDE  SYNTHESIZER

多尺度多肽合成儀

7 EASY STEPS TO PEPTIDE SYNTHESIS USING PUREPEP CHORUS

7個簡單的步驟合成肽使用PUREPEP CHORUS

Step 1: Group reaction vessels to run simultaneously or sequentially. Different groups can run unique protocols.  

步驟1:將反應(yīng)容器分組，同時或順序運行。 不同的組可以運行唯一的協(xié)議。 
 

Step 2: Input parameters including coupling methods, reagent excess, and information on resins for use in record-keeping and calculations.

步驟2:輸入?yún)?shù)，包括偶聯(lián)方法，試劑過量，以及用于記錄和計算的樹脂信息。

Step 3: Add peptides from the library or create new ones with the sequence editor. Select cycles for special chemistry within groups and prime-free Single-ShotTM additions for expensive reagents.  

步驟3:從庫中添加多肽或用序列編輯器創(chuàng)建新的多肽。 選擇循環(huán)組內(nèi)的特殊化學和無底質(zhì)的single-shotTM添加昂貴的試劑。
  

Step 4: Choose to use default bottle positions for amino acids and reagents or arrange as desired.  

步驟4:選擇使用氨基酸和試劑的默認瓶位或按需要排列。

Step 5: Assign pre-programmed or custom chemistry protocols to any cycle in any group. For heated syntheses, select default temperatures for deprotections and couplings, and exceptions for any step, cycle, RV or group.

步驟5:將預(yù)編程或自定義的化學協(xié)議分配給任何小組的任何循環(huán)。 對于加熱合成，為脫保護和耦合選擇默認溫度，并為任何步驟、周期、RV或組選擇例外溫度。

Step 6: Let the software calculate the weights and volumes needed for all reagents, as well as theoretical yield and estimated synthesis time.

步驟6:讓軟件計算出所有試劑所需的重量和體積，以及理論產(chǎn)率和估計合成時間。

Step 7: Start synthesis from the beginning or at any cycle and step desired. View UV and temperature data in real time while a synthesis is running, and follow countdown to completion of your peptide.

步驟7:從一開始或在任何所需的循環(huán)和步驟開始合成。 查看紫外和溫度數(shù)據(jù)實時合成運行，并遵循倒計時完成您的肽。

IntellisynthTM UV Monitoring

IntellisynthTM紫外線監(jiān)控

Proprietary Intellisynth real-time UV monitoring can be configured on as many reaction vessel pairs as you desire, enabling automated optimization of the deprotection reaction. Monitoring the solution during mixing allows control of both reaction times and the number of repetitions, ensuring deprotections go to completion while avoiding wasted time or reagent. 

Intellisynth實時紫外監(jiān)測可以配置在任意多對反應(yīng)容器上，實現(xiàn)去保護反應(yīng)的自動化優(yōu)化。 在混合過程中監(jiān)控溶液可以控制反應(yīng)時間和重復(fù)次數(shù)，確保去保護完成，同時避免浪費時間或試劑。  

View real-time UV data while a synthesis is running and full data from any completed synthesis.

查看實時紫外數(shù)據(jù)，而合成運行和完整的數(shù)據(jù)從任何完成的合成。

Individual graphs show reaction progress during a cycle.  Summary graphs indicate differences in times and repetitions between different cycles, providing valuable diagnostic information on synthetic trouble spots for difficult sequences.

單個圖表顯示了一個周期內(nèi)的反應(yīng)進展。 摘要圖顯示了不同循環(huán)之間的時間和重復(fù)的差異，為復(fù)雜序列的合成故障點提供了有價值的診斷信息。  

Induction heating

感應(yīng)加熱

Induction heating can be configured on as many reaction vessel pairs as you desire. It accelerates chemistry and improves purity for difficult sequences. Heating conditions in each of the six reaction vessels can be independently set to enable temperature scans for process optimization or to accommodate parallel synthesis of sequences requiring different protocols. View real-time temperature data while a synthesis is running and full profiles from any completed synthesis.

感應(yīng)加熱可以配置在你想要的許多反應(yīng)容器對。 它加速了化學反應(yīng)，提高了復(fù)雜序列的純度。 六個反應(yīng)容器中的加熱條件可以獨立設(shè)置，以進行溫度掃描，以進行工藝優(yōu)化，或適應(yīng)需要不同方案的序列的并行合成。 在合成過程中查看實時溫度數(shù)據(jù)，以及任何已完成合成的完整剖面。  

21 CFR part 11 compliance and IQ/OQ/PQ

PurePep software has been designed with 21 CFR part 11 compliance in mind, ensuring the instrument can be used in highly regulated cGMP laboratory environments. IQ/OQ support and PQ guidance are also available.

PurePep軟件設(shè)計時考慮了21 CFR part 11的遵從性，確保儀器可以在高度規(guī)范的cGMP實驗室環(huán)境中使用。 IQ/OQ支持和PQ指導也是可用的。 

Key software features include:

主要軟件功能包括:

User management用戶管理

Electronic signatures電子簽名

Audit trail審計追蹤

Easy-to-review reports for maximum traceability易于審查的報告，最大的可追溯性
  

PURITY: PurePep Pathway dedicated chemically inert pathways 

PRODUCTIVITY: flexible configuration and in-lab upgradeable 2, 4, 6 RVs 

POWER: independent, controlled induction heat and real-time UV monitoring

純度:purepp途徑專用的化學惰性途徑  

生產(chǎn)力:靈活的配置和實驗室可升級的2、4、6RVs  

電源:獨立、可控的感應(yīng)加熱和實時紫外監(jiān)測 

Purity starts with PurePep? pathway inside with proprietary microfluidics that minimize cross-contamination, dead volumes, and reagent carryover. 

純度從PurePep?通路內(nèi)部開始，使用專有的微流體，最大限度地減少交叉污染、死體積和試劑攜帶。  

SYSTEM SPECIFICATIONS系統(tǒng)主要技術(shù)指標

Number of reaction vessels：2, 4, 6 configurable with in-lab upgrades

反應(yīng)容器數(shù)量：2，4，6可通過實驗室升級進行配置

Synthesis scale range：0.005 - 1.0 mmol per reaction vessel (6.0 mmol total for a 6 RV system) 

合成刻度范圍：0.005 - 1.0 mmol /個反應(yīng)容器(6 RV系統(tǒng)總濃度為6.0 mmol) 

Reaction vessel volume：Plastic, disposable - 10 mL, 45 mL；Borosilicate glass - 10 mL, 25mL, 40 mL

反應(yīng)容器體積:塑料，一次性- 10ml, 45ml;硼硅酸鹽玻璃- 10ml, 25mL, 40ml  

Number of solvent positions：8 solvents (1 to 4 L each standard, larger vessels available) 10mL, 25mL or 45 mL

溶劑位置的數(shù)量:8溶劑(1到4 L每標準，更大的容器可用)10mL, 25mL或45ml 

Number of amino acid positions：27 amino acid bottles or Single-Shots plus six Single-Shot only positions for specialty reagents 10 mL (for standard or Single-Shot delivery for specialty reagents), 50 mL (Single-Shot only positions), 120 mL, and 400 mL sizes.

Pre-packaged and empty bottles available

氨基酸位置的數(shù)量:27個氨基酸瓶或單針加6個單針專用試劑位置10ml(用于標準或?qū)Ｓ迷噭┑膯未巫⑸?，50ml(僅用于單次注射位置)，120ml和400ml大小。可提供預(yù)包裝和空瓶

Chemistries supported：Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA  

支持的化學:Fmoc, t-Boc，有機，類肽，組合，分支，PNA 

Activation：in situ and pre-activation

激活:原位和預(yù)激活

Fluid transfer method：Positive pressure with nitrogen

流體輸送方法:正壓氮氣輸送

Agitation method：Nitrogen bubbling - adjustable, and/or optional

攪拌方式:氮氣鼓泡-可調(diào)，和/或可選 

Mixing method：oscillating shaker mixing

混合方法:振蕩式振動篩混合

Heating method：Controlled induction heating, configurable to the number of RVs

加熱方式:可控感應(yīng)加熱，可根據(jù)RVs數(shù)量配置

Cleavage:Automated on-board cleavage, separate fluid path and vent

解理:自動板載解理，獨立的流體通道和排氣口

Waste container:(1) 20 L D.O.T rated container with over-flow sensor in cap 

廢物容器:(1)20升的額定運輸量容器，瓶蓋上有溢出傳感器

Reporting:Real-time log of each instrument function, print to file or printer. Includes e-mail notifications to send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or if an error occurs.

報告:實時記錄各儀表功能，打印到文件或打印機。 包括電子郵件通知，用于在指定的程序步驟、每個周期的開始或發(fā)生錯誤時向計算機或手機發(fā)送電子郵件。

Power:100-230VAC , 50/60Hz

電源:100-230VAC , 50/60Hz

Dimensions：87.1 cm x 72.1 cm x 72.4 cm (34.3” x 28.4” x 28.5”)

尺寸：87.1 cm x 72.1 cm x 72.4 cm (34.3” x 28.4” x 28.5”)

Weight：90.7 kg (200 lbs)

重量：90.7 kg (200 lbs)

Warranty：One year, parts and labor

保修:一年，包括配件和人工