為什么使用熱循環(huán)器?  

實驗室使用熱循環(huán)儀(或熱循環(huán)儀、PCR儀、DNA放大器)，通過兩個極端溫度的重復循環(huán)來放置樣本。 它常用聚合酶鏈反應(PCR)來擴增DNA片段。 熱循環(huán)器也用于實驗室進行其他溫度敏感反應，包括限制性內切酶消化和快速診斷。 

它的工作原理是精確地提高和降低其熱塊的溫度。 樣品(在管中)放置在塊中的井中，由塊進行加熱和冷卻。 

在熱循環(huán)器中尋找什么  

對于聚合酶鏈反應和其他用途，熱循環(huán)器必須能夠在每次運行中為每個樣品提供完全相同的溫度變化。  

溫度均勻性-在整個區(qū)塊內提供相同的溫度，區(qū)塊中間或邊緣的樣本以及整個測試過程的結果都是相同的。  

溫度精度-確保精確和穩(wěn)定，可重復的溫度在運行的時間內，對每一個樣品在塊。  

在開式抽屜操作期間的均勻性和準確性-使移液和酶導入無論何時需要，效果可以忽略不計。  

快速均勻的溫度變化(斜坡速率)-在保持整個區(qū)塊溫度均勻的同時，盡可能快地達到所需的溫度。  

樣品完整性和減少污染-在運行過程中通過封閉和密封批次。  

最小化啟動和啟動時間-結合快速的斜坡速度，使實驗室每天可以完成更多批次。  

占地面積小-提供所需的性能，同時占用甲板上盡可能少的空間。  

靈活定位-由于熱循環(huán)器的排氣或甲板臂的阻礙，放置位置幾乎沒有限制。  

使用INHECO ODTC?熱循環(huán)器  

ODTC?用于PCR過程(聚合酶鏈反應)，在樣本中擴增基因序列。 擴增遵循指數(shù)曲線，因此在幾次溫度循環(huán)后，樣品中出現(xiàn)幾百萬次重復。 各種檢測方法都需要這個很高的數(shù)字，因為測量/檢測系統(tǒng)的傳感器只有在有足夠的信噪比時才會響應。 

該ODTC?也用于NGS(下一代測序)。 基因片段的讀取，即堿基對(ATGC)發(fā)生的序列，是由測序器進行的，但為了能夠處理樣本，需要使用PCR制備(或富集)該基因片段的樣本。 

ODTC?是理想的基因分析。 由于基因分析在更多的診斷程序和研究工作中使用，它需要自動化，ODTC?是在高度自動化的環(huán)境中優(yōu)化使用。

Why use a thermal cycler?

Labs use a thermal cycler (or thermocycler, PCR machine, DNA amplifier) to put samples though repeated cycles of two temperature extremes. It is frequently used to amplify segments of DNA using the polymerase chain reaction (PCR). Thermal cyclers are also used in laboratories to enable other temperature-sensitive reactions, including restriction enzyme digestion and rapid diagnostics.

It works by precisely raising and lowering the temperature of its thermal block. Samples (in tubes) sit in wells in the block and are heated and cooled by the block.

What to look for in a thermal cycler

For PCR and other uses, the thermal cycler must be able to deliver exactly the same changes in temperature for every sample in a run, and for every run.

Temperature uniformity – delivering identical temperature across the entire block, with identical results for samples in the middle or on the edge of the block, and throughout the run.

Temperature accuracy – ensuring accurate and stable, reproduceable temperatures for the duration of the run, and for every sample in the block.

Uniformity and accuracy during open-drawer operations – enabling pipetting and enzyme introductions whenever needed, with negligible effect.

Rapid uniform temperature change (ramp rates) – reaching required temperatures as rapidly as possible whilst maintaining temperature uniformity across the entire block.

Sample integrity and contaminant reduction – by enclosing and sealing the batch during the run.

Minimized start-up and boot times – combined with rapid ramp rates so laboratories can complete significantly more batches per day.

Small footprint – delivering the required performance while taking up as little space on deck as possible.

Flexible positioning – with few, if any, restrictions on placement due to the thermal cycler’s air venting or obstruction of the deck arm.

Use of the INHECO ODTC? thermal cycler

The ODTC? is used in the PCR process (polymerase chain reaction), to amplify a gene sequence in a sample. The amplification follows an exponential curve, so that after several temperature cycles several million replicates are present in the sample. This high number is required for various detection methods, as the sensors of the measurement/detection systems only respond when there is sufficient signal-to-noise rate.

The ODTC? is also used for NGS (Next Generation Sequencing). The reading of the gene segment, i.e., the sequence in which the base pairs (ATGC) occur, is carried out by the sequencer, but in order for this to be able to process the sample, it’s necessary to prepare (or enrich) a sample of the gene segment using PCR.

The ODTC? is ideal for gene analysis. As gene analysis is used in more diagnostic procedures as well as in research work it requires automation, and the ODTC? is optimized for use in highly automated environments.